Part:BBa_K1595029:Design

EDA and GSG linker

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 729
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 641
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Added GSG so EDA could easily be linked to it's associated peptide in future use.

EDA had an internal PstI cutsite that was removed by altering the DNA sequence.

Source

Synthesized by DNA 2.0

References

EDA: NCBI accession number P0A955